KMID : 0379119980260030314
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Korean Journal of Mycology 1998 Volume.26 No. 3 p.314 ~ p.320
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Isolation and Characterization of a Wound or UV Induced cDNA Fragment from Pleurotus sajor - caju
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¹Ú¼öö/Park, Soo Chul
·ùÁøâ/À¯¿µº¹/Á¤¹ÌÁ¤/Á¤¿íÁø/±è¹ü±â/Ryu, Jin Chang/Yoo, Young Bok/Jeong, Mi Jeong/Jung, Uk Jin/Kim, Bum Gi
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Abstract
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A 0.4 kb cDNA fragment was isolated from mRNA of UV or mechanical wound damaged Pleurotus sajor-caju by the differential display method. Expression of the gene corresponding to this cDNA fragment was highly induced by mechanical wound damage or UV treatment. This gene showed only basal level expression in mycelia, stipe, and cap under normal growth conditions. Sequencing analysis showed that this cDNA fragment contains partial open reading frame. Homology search using genbank database revealed that although this gene do not have homology with already reported wound induced genes, it has a significant sequence homology in defined region with the cdc2-related protein kinase gene which is known to be involved in negative regulation of meiotic maturation in Xenopus oocytes.
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